PCR is a technique used to amplify target DNA in a sample. It’s a well-known method that has undergone numerous modifications to enhance its capabilities. This year, it’s turning 40 years old. PCR has ...

The key to an optimal PCR experiment is primer design. In general, primers should be between 18 and 24 nucleotides long and have a GC content between 40 and 60 percent. Ideally primers should contain ...

This guide provides a comprehensive overview of PCR technologies, highlighting the benefits, workflows and applications of endpoint PCR, qPCR and dPCR. Using gene expression profiling as a central ...

When reading articles about genetic testing, terms like PCR, Sanger sequencing, NGS, and digital PCR often appear.While all ...

What is the role of the polymerase chain reaction (PCR) process in the diagnosis of abdominal tuberculosis (TB) and in differentiating it from Crohn's disease? Tuberculosis may involve any part of the ...

GeneFisher—fishing for genes, perhaps? Fishing genes out of genomes? Just fishing? The subtitle, Interactive PCR Primer Design, “hooked me” (get it?) but it shouldn’t have in retrospect. After all, ...

Fungi are frequently found both around and within plant tissues (especially in roots) and are involved in both plant nutrient acquisition and resistance to pathogens. Thus, characterizing the ...