As analytical technology continues to advance, both regulatory agencies and pharmaceutical companies are keen to avail themselves of these enhancements. In the case of chromatography, evolution from ...

Given the nature of biomolecules, such as amino acids and peptides, many HPLC reversed phase bio-separations often require ion-pairing reagents, such as Trifluoroacetic acid (TFA), in combination with ...

Operating HPLC at higher than ambient temperatures can improve peak shapes and enable faster run times. Preheating the mobile phase is significant in high temperature liquid chromatography as the ...

The authors discuss the approach taken to develop a new calibration approach, its associated protocols, and how it can be used to calculate data. The use of open-access high-performance liquid ...

Gradient elution involves a programmed change in mobile-phase composition over time, transitioning from weak to strong during a separation. Linear gradients, characterized by a constant change in ...

High performance liquid chromatography (HPLC) is also called "high-speed liquid chromatography", "high-resolution liquid chromatography", "modern column chromatography" and so on. High performance ...